A Technique for Measurement of Methylmalonic Acid in Cattle Urine
نویسندگان
چکیده
منابع مشابه
Colorimetric method for estimating methylmalonic acid in urine.
The increased urinary excretion of methylmalonic acid in vitamin B12 deficiency has attracted attention and several studies have suggested that it has clinical value as a test of vitamin B12 deficiency (Cox and White, 1962; Bashir, Hinterberger, and Jones, 1966). Chromatographic methods of estimation have been used but are either time consuming or only semiquantitative over a narrow range of me...
متن کاملGas-chromatographic determination of methylmalonic acid in urine and serum.
We describe a gas-chromatographic procedure for determining methylmalonic acid in serum and urine. The sample is acidified and the compound is extracted from it with diethyl ether, derivatized with N,Obis(trimethylsilyl)trifluoroacetamide, and gas chromatographed. Precision and sensitivity of the method were verified by recovery studies and by comparing the resultswith those foran established p...
متن کاملAnalysis of dicarboxylic acids by tandem mass spectrometry. High-throughput quantitative measurement of methylmalonic acid in serum, plasma, and urine.
BACKGROUND Methylmalonic acid (MMA) is a dicarboxylic acid whose concentration can be increased in blood and urine in patients with an inborn error of metabolism or vitamin B(12) deficiency. We developed a method for the selective analysis of dicarboxylic acids that exploits the high specificity of tandem mass spectrometry (MS/MS) and the substantial difference in fragmentation patterns of the ...
متن کاملDEVELOPMENT OF A METHOD FOR THE DETERMINATION OF 4-HYDROXY-3-METHOXYMANDELIC ACID (VANILLYLMANDELIC ACID) IN URINE BY PAPER CHROMATOGRAPHY
We have developed a simple and precise paper chromatographic method for the determination of 4-hydroxy-3-methoxymandelic acid (VMA) in urine. Concentrations of VMA in patients with neuroblastoma were increased in comparison to controls. The linearity was excellent in the concentration range tested. The within-assay coefficient of variation for control and patient urine was less than 2.2%. ...
متن کاملMethod for quantitative measurement of xanthurenic acid in urine.
A method is reported for determining urinary xanthenuric acid (XA), a meta. bolite of tryptophan. XA is extracted from urine with isobutanol (2.methyl. 1.propanol), transferred to ammonium hydroxide, and separated from other urinary metabolites on thin.Iayer cellulose plates with use of 0.2 acetate buffer, mol/liter, pH 5.4. The XA is measured spectrophotometrically after diazotization with 2-c...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1977
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(77)84106-4